Continuity mistake: At the beginning of the movie, we see, from a POV shot, Blade running towards a road when the car carrying the two assassins pulls up in front of him and the two men get out. The shot then changes to an overhead view of the car. Blade should be visible in front of the car but isn't. (00:04:46)
Continuity mistake: When Andre Toulon puts Blade into the suitcase, near the start of the film, Blade's hook is on his right hand but it is attached to his left hand in all other shots of him. (00:09:45)
Continuity mistake: When Frank and Carissa are having sex and Carissa notices the door is open, her hair moves from hanging over her shoulder to behind her shoulder. (00:49:15)
Continuity mistake: Toward the end when Alex is being punched by the "Puppet Master" his mouth is all bloody. Then Megan smashes a vase on The Puppet Master's head, and in the next shot Alex's face is no longer bloody. In the next closeup of him the blood has returned. (01:13:05)
Continuity mistake: When Andre puts Blade in the suitcase, Blade's knife is missing and there's nothing where his left hand should be.
Continuity mistake: When Tunneler drills the back of Neal's leg, you can see blood in the wound, but when Neal pulls him out, he has green ooze on his drill instead of blood.
Continuity mistake: When Blade gets on the elevator in the beginning, pay attention to the legs of the woman in the elevator. When she gets on, she is wearing black stockings. When she gets off, she is wearing cream-colored stockings. The rest of her clothes that are visible are the same (same shoes, skirt, etc.), as is the wardrobe of the man who gets on and off with her, so it's meant to be the same woman. (There's also no indication that the elevator made any stops on the way up).
Continuity mistake: When Blade is knocking on Toulon's door, his hook is sideways, in the next shot, his hook is pointing down.
Continuity mistake: When Blade's dragging the chair from Dana's door to Frank & Carissa's door, in the shot facing Blade his hook's above the crossbars of the chair leg, in the shots before and after, his hook's about two inches under the crossbars.