Revealing mistake: Halfway through the movie, Alta is swimming in the pond, supposedly nude. However, when she climbs out, she can be seen wearing a flesh-covered swimsuit. (00:45:15)
Suggested correction: Alta never said she was naked. She didn't know what a bathing suit was and Adams assumed she was naked.
Other mistake: When the Id creature is melting through the door and Morbius is denying he subconsciously created it, the Commander draws his blaster giving the impression he will kill Morbius to save them if necessary. But Robby deactivated the blaster just a few scenes prior. Was he going to beat Morbius to death with it?
Suggested correction: That was a moment of desperation, so he wasn't thinking, and, well, yes anyway.
Character mistake: When Adams is chewing out Altaira for flirting with his crew, he says that he is "in command of 18 perfect physical specimens." With him, that would make for a total crew of 19 on the C57-D. But the complete cast only shows 18 names, including Walter Pidgeon as Morbius and Anne Francis as Alta. (And discounting Robby and his human handlers). Thus it would seem Adams is off by three. He has only 15 under his command. I've tried counting them when they're in group shots, but can't see everyone.