Factual error: When Pr Langdon and Dr Brooks arrive in Padova by train from Florence, they ran next to a sign which says "Padova Centrale." However, there is no such sign in the Padova train station with the writing "Centrale", only "Padova." Moreover, on platform 8 you see a Freccia Rossa, a kind of train that in Padova only passes through platform 1, 2, 3 and 5. (01:11:30)
Factual error: In Istanbul the caption reads "7:53 PM" but shadows shows it is actually between 1:00 PM to 2:00 PM. (01:37:50)
Factual error: When Langdon finds the bio tube in his jacket and opens it, it finds a "bone" cylinder in it, which Dr Brooks immediately identifies as human. There is no way to tell if a crafted and modified bone is human or animal by simply looking at it.