Continuity mistake: In the scene where after Peter gets his diploma and has a chat with Gwen, he is carrying a backpack on both shoulders. As the scene continues it switches to one shoulder then both several times. (00:15:50)
Continuity mistake: When Aunt May takes a picture of Gwen and Peter she places two left fingers on the camera. When the angle changes there's just one. (00:15:50)
Continuity mistake: After Aunt May takes a picture of Gwen and Peter they're standing side by side. A shot later they're a meter and a half away from each other. (00:15:55)
Continuity mistake: After graduation, when Peter kisses Gwen he places his hand on her cheek. When the shot changes the hand is gone. Also, her hair is covering her cheek or perfectly brushed depending on the angle. (00:16:25)
Continuity mistake: When Gwen is summoned by her parents to take a graduation family picture she says to Peter she'll work "all night long" and places her left hand on Peter's neck. When the shot changes her hand is gone. (00:16:30)
Continuity mistake: Coming out of the Chinese restaurant, Emma Stone is closing her coat, and you can see in the view from behind that she even completes the movements to properly tie the belt. The scene continues, the belt is perfectly fitting and closed, but the last shot of the scene shows Gwen Stacy in front of the door with the coat open and the belt loose. (00:17:35)
Continuity mistake: The little bullies are pushing the wind turbine kid against the fence; they literally have their fingers inside the links; they look up and notice Spidey, barely move very slowly, but a moment later they are much further back in the alley giving him a solid few meters of space where he lands. (00:20:35)
Continuity mistake: When the Statue of Liberty tells Spidey "Nice outfit!" the people in the background are in different positions at the cut. (00:21:20)
Continuity mistake: When Peter pulls the boot of the costume under the cover to prevent May from seeing it, you can get a good look at the stack of books he's going to trip into a few seconds later. In fact, you can still see even as he trips, that the stack includes the famous leather bag from his father. He doesn't knock it to the floor when he trips, but the next shot just shows it on the floor anyway. (00:23:25)
Continuity mistake: When Aunt May is about to do the laundry she gives Peter a sandwich with a large piece of lettuce in it, but in the following shots the lettuce disappears. (00:26:40)
Continuity mistake: When Max is talking to his boss and fantasizes to assault him, there's a woman walking past them reading some documents, and she keeps coming from the corridor and passing them by - that's without considering Max's fantasy, just shots of the 'real' events. (00:27:50)
Continuity mistake: When Max holds the cables and sings happy birthday to himself, the position of his left-hand changes, from more distant to the ring of the connector in the view from underneath, and closer to it in the other angle. (00:36:10)
Continuity mistake: At the board meeting, Harry is playing with his father's electronic seal in a way that displays towards the camera the side with the Oscorp logo. But there are two different camera angles from opposite sides, it can't be facing both at the same time. (00:36:55)
Continuity mistake: When Harry at the board meeting sarcastically paraphrases the Connors incident mentioning the "giant lizards" you can see Felicia Hardy in background is writing down things, but she isn't in the previous shot. (00:37:05)
Continuity mistake: At the meeting, Harry says "What was Dad thinking?" The lawyer with the purple bowtie is looking at him, hands off the table, but in the next shot he has both hands on it. Same a few seconds later when Harry says "Hi" to Felicia; hands on the table first, off the table in the reverse angle. (00:37:35)
Continuity mistake: Harry Osborn says at the business meeting that everyone will work for Felicia; Felicia's hand position in close-up is egregiously different from the one she has in the view of the table. (00:38:30)
Continuity mistake: When Peter and Harry chat by the river and Peter jumps to the other side of the railing, his left hand is either atop or below the railing, depending on the shot. (00:41:30)
Continuity mistake: The two friends are chatting from opposite sides of the riverside railing. Peter says "Yeah"; look at the folded hat in his pocket. Next shot, he adds "I don't know" and the cloth is wrapped in a different way than before. (00:41:30)
Continuity mistake: Playing with stones by the river (the GPS coordinates on scree are a perfect match for Pebble beach), Harry says "I tried to forget everything about this place." Peter is scratching his chin, but in the next shot his hand is nowhere near his face or even in frame. (00:42:05)
Continuity mistake: When Peter spots Gwen on the street he stops a truck that stops 20 cm away from him. When the angle changes the truck is further back. (00:45:40)