Revealing mistake: When the truck starts rolling back you can see tyre tracks on the grass from previous takes. (00:46:35)
Revealing mistake: Just the bridge is blown up at the start, you can see all the soldiers have been replaced by dummies. (00:12:10)
Revealing mistake: When FDR jumps in the Beetle to help Lauren you can see it's a stunt making the jump - his hair is darker.
Revealing mistake: The SEALs assaulting Bin Laden's compound are using infrared lasers on their rifles, which can only be seen under night vision. The lasers being AN/PEQ-15s which the SEALs have attached to the top of their rifles. However, most if not all of the rifles have the laser beam coming from the bottom left of the hand guard, not from the top mounted laser unit. It's possible the/PEQ-15s were faux units and different lasers mounted to the rifles were used to give the effect.
Revealing mistake: When Niles York first appears on camera beside the plane bearing his name, most won't notice, but you can see the pilot's hatch open, revealing that it is not a passenger jet, but a fighter plane. You can also see the air intakes on the wing, further evidence that this is not a passenger or private jet. (00:02:11 - 00:22:18)
Revealing mistake: When Luke is signing the receipt for his new clothes, the amount on the CC receipt is less than $10.00. (00:40:16)
Revealing mistake: In the lead up to the final scene, prior to the cranes being in alignment, when Spider-Man is jumping between buildings, he falls down to a fire escape, and you can see that the bars or rails on the railing he lands next to are not made out of metal as they easily move as the actor or stuntperson moves next to them. (01:48:42)