Continuity mistake: In the fight between Barkis and Victor in the church, Victor slashes at Barkis with the fork and leaves 3 very noticable tears in Barkis' jacket. These tears aren't seen in any of the subsequent shots. (01:09:15)
Continuity mistake: When drunken Stewie is talking at the breakfast table to Brian, Brian has bacon and only one egg on his plate. After Stewie leaps from the shelf, Brian suddenly has two eggs.
Factual error: When Chicken Little is in his bedroom, after he scored during his baseball game, he looks into the spoon he is using as a microphone to see his reflection. He looks into the side that is curved away from you. When you look at that side of a spoon your reflection is upsidedown, but Chicken Little's was right side up.
Continuity mistake: When Alex tries to get Melman out, he is lying near the sea, but when Alex tries to get him out with the tree, he is farther away from the sea.
Continuity mistake: Japhet the goat steps on a chair but it disappears in the next shot.