Continuity mistake: When the Coyote is pursuing the Road Runner at the start of the cartoon, he is holding a fork in his left hand and a knife in his right. But when we see the Coyote a few seconds later, he is now holding the knife in his left and the fork in his right.
Continuity mistake: When Jerry is removing the nail from Spike's foot with a hammer, the distance of the wall behind Spike changes between shots.