Character mistake: During the meeting between Langdon, Sophie and Sir Teabing, Teabing refers to Scatoma as "the mind seeing what it wants to see." Scatoma refers to a partial loss of vision. He probably meant to use Pareidolia, which refers to seeing patterns that are not really there.
Character mistake: When Nicholas Cage bumps into the beehive, he sees the bees coming towards him and runs towards all the other beehives. Given that he bumped into the beehive on the outer edge, it would have been safer, quicker and much less risky to run back the way he came to escape than run towards all the other bee hives. To complicate things Cage knew he was severely allergic to bees.
Character mistake: The Daily Mirror, at the beginning of the movie, reports the fatal accident of the soccerplayer with the spelling "fatale".