Factual error: In the sequence where the animals get on the first subway, it says they are on the 59th St subway. But, there is no 59th St subway in NY. The only subway station close to 59 is the 57th St subway.
Suggested correction: While the other correction lists a 59th street, that's in Brooklyn. There is one in Manhattan though, 59th - Columbus Circle, which is also the south-west corner of Central Park.
Suggested correction: There is definitely a 59th street subway station: https://en.wikipedia.org/wiki/59th_Street_ (BMT_Fourth_Avenue_Line).
Factual error: When Chicken Little is in his bedroom, after he scored during his baseball game, he looks into the spoon he is using as a microphone to see his reflection. He looks into the side that is curved away from you. When you look at that side of a spoon your reflection is upsidedown, but Chicken Little's was right side up.
Factual error: During the bike chase towards the end, as Cloud and Loz/Yazoo crash through the security gate, you can see that the lettering is misspelled: "Authorized Parsonnel Only." It should read "Authorized Personnel Only."