Continuity mistake: When the Nazi is strangling Indy with the chain, Indy looks down into the porthole of the tank and yells, "Dad!" Before he throws the gun down into the tank Indy's hat comes loose, but when the next shot is back on Indy, and he yells, "Dad! Dad, get out!" now his hat is back to normal.
Visible crew/equipment: After Elsa gives Donavan the false Grail, he turns and you can see the reflection of the camera lens in the cup. (01:51:45)
Continuity mistake: When we first see the knight guarding the Grail, his cloak is tied off with a braided rope, which hangs down on his right side. It stays this way until he says "You have chosen wisely," where the rope has somehow shifted to his left side. The rope goes back to normal when the knight waves goodbye at the end of the movie. (01:49:10 - 01:54:05)
Continuity mistake: After Sr. says "Let it go" as Indy is trying to get the grail, Indy is swinging and not being supported by the wall. The next camera angle shows him grasping the wall near his head. Switched back to Sr.'s view, he is again not holding the wall.
Continuity mistake: When the white robed men ambush the Nazis just after they blow up Salah's car, there's a shot over a Nazi's shoulder as he fires at one of the white robed guys running up a hill. The Nazi's machine gun is held straight out and doesn't move but the dust that flies from the squibs suggest that he fired as he moved the gun right to left which he did not do. The odd thing is that AFTER the squibs go off, the Nazi turns his gun to the left. (01:25:55)
Factual error: When Hitler signs the Grail Diary, in the screen and VHS release he signs Adolph, not Adolf. This mistake was corrected in the DVD release. (01:07:35)
Factual error: When Indy and his Dad are in Berlin, there is a big book burning going on. But the only book burning that was held in Berlin was May 10th in 1933 - 5 years before the Joneses visit it in search for the Holy Grail. (01:07:50)
Continuity mistake: When Indiana is drinking champagne with the businessman in the fancy apartment he holds his glass by the stem in one shot and then by the bowl in the next shot. As the conversation continues the glass goes up and down in Indiana's hand like a yo-yo.
Continuity mistake: When Indy and Vogel are fighting atop the tank we see the soles of Vogel's boots and there is a red hexagon dot under each heel, but while Indy is hanging off the tank's side mount gun the soles of Vogel's boots are different.
Continuity mistake: On the tank a soldier jumps from Indy's right to attack him. The following angle shows the soldier coming from the front.
Continuity mistake: When Indy and Elsa are wading in the petroleum looking for the knight's tomb, Elsa unbuttons and opens her jacket and starts to take it off. In the next shot she opens her jacket and takes it off again. (00:34:15)
Other mistake: On the zeppelin, Indy's dad says, "You left just when you were becoming interesting..." Harrison Ford is mouthing Sean Connery's lines. (01:14:50)
Continuity mistake: In the beginning where Indy is young, he fights some guys on that zoo train. When he's on the crate with the rhino in it, the background changes in each shot. Once there are trees behind and a split of a second later there are rocks, once there's a cliff and once a plain, and it all changes in splits of seconds as they change shots. (00:07:25)
Continuity mistake: When the Nazi Colonel discovers Henry Jones sen. aboard the zeppelin, Indy asks him for his flight ticket. In the first shot, Indy is standing right beside the colonel (the camera is behind the colonel and Indy comes in from the right), in the subsequent shot, Indy is standing behind the colonel.
Continuity mistake: While in the Germans tank, the right side of Marcus's jacket is bent and wrinkled. A Frame later it looks perfect.
Continuity mistake: Indy and co. leave their horses a way from the steps to the temple, but when we cut to a closer shot they're very close to the entrance.
Continuity mistake: In the Grail temple, when Salah says "Drop your guns please," he is holding a rifle. When Indy instructs his father a few seconds later to get up, Salah is not holding a rifle but has his hands crossed.
Continuity mistake: In the scene in the temple after the two soldiers attempt to get past the first task, the first soldier is seen on his back holding his head beside him. When Indy attempts the first task, both dead soldiers are on their stomachs with no heads in sight.
Visible crew/equipment: When Indiana and the Protector of the Grail are fighting in the boat that is about to be chewed up by the giant screw/propeller, when the angle changes to the top point of view, you can see the rope pulling the front of the boat around to position the rear for destruction... (00:39:10)
Continuity mistake: At the airport, someone is reading a newspaper. Instead of the year 1938, the date of the newspaper is 1918 (a guess: I think this mistake happened because the art director wrote the date in a rush, stretching the "3" in a way it seemed a "1" when the people of "atrezzo" saw it).